DNA microarray technology and bioinformatics have enabled some advances in the understanding and manipulation of gene behavior in simple, developmental models. These advances have medical implications, especially with regard to the treatment of carcinogenesis.
There remains, nevertheless, major problems, such as those that pertain to the genomic control of mutation --- especially of hypermutation --- which prevent a complete understanding and hence a comprehensive, effective application of the new biotechnologies and the imaginative creation of new ones. This is especially the situation in the context of investigating, unstable gene behavior within complex, developmental systems, both in vivo and in vitro.
One can reconceptualize biological investigations / experiments in order to solve these problems.
Through my extensive and diversified training and experience in the genetic / epigenetic control of mutation and in vitro development, as well as in its theoretical aspects, I can define those problems and provide in depth solutions, which have significant implications for developmental genetics.
In general, such solutions would require new, related paradigms, suggesting investigations along new avenues. This would result in comprehensive, in depth, and thus effective, medical and agricultural applications.
As a Consultant to certain companies or laboratories, I could comprehensively illustrate the problems and in turn develop solutions---perhaps in collaboration with scientists who would be open to exploring new paradigms---that would ultimately give forth powerful applications.
With regard to in vitro developmental systems, I have a broad background in various facets of genetics, including the transgenic transformation of plants for disease resistance.
My specialty
has been in developing procedures for overcoming recalcitrant organogenesis and embryogenesis from plant tumor (callus) in tissue culture of various plant species, including trees, an important requirement, for recovering transgenic plantlets by way of transformed callus in vitro. Pine, bean, and tobacco were amongst the plants investigated in tissue culture by this author (Lieber, 1980a, 1995, 1996).
The photographs (Figures 1a thru 6) are examples of my experiments leading to successful plantlet, bud, and embyogenic development at high frequency in vitro, where factors inhibiting in vitro development were repeatedly overcome.
Such experiments are the results of a successful application of a principle within a comprehensive theory pertaining to non-uniform forces (stress) and their role in biological development (Lieber, 1996, 1998a, 1998b, 1998c, 2000, 2001a, 2001b) [ and weblinks].
This principle becomes apparent, among other ways, through the manifestation of a universal, dimensionless biological constant, first noted by the author (Lieber, 1998a)
This constant, numerically equivalent to the Golden Ratio, 1.618, was found to compose the dimensional constants of physics. Among other matters, this constant defines or manifests an underlying developmental, dynamical pattern in all physical and biological phenomena.
For example, this dimensionless constant is readily seen as contributing to the
Planck Length. The latter is a dimensional constant which refers to the connection between gravitational force and quantum processes in a nearly infinitesimal region of space-time and suggests, because of its dimensionless component, a particular
dynamic pattern, a spiral generation, operating through that connection.
On the much higher organizational level or scale of biological phenomena, this dynamical pattern is readily manifested in the spiral/vortical morphology of many plants and animals, and their parts, e.g., pinecones. Photographs (Figures 9a thru 14) show this particular morphology.
Adaptively responsive hypermutation to stress is a phenomena that is also predicted by the principle and its biological constant. Using the fungus Aspergillus nidulans, experimental conditions and genetic strains were created by the author that demonstrated this type of mutagenesis in the fungus and its importance for development (Lieber, 1975c, 1976b, 1998b) [and weblinks].
The importance of this research was described in a personal letter by the Nobel Laureate, Dr. Barbara McClintock. Photographs show some examples of this phenomenon (Figures 7 & 8 ).
Later research by the author demonstrated adaptively responsive hypermutation to stress (non-uniform force-magnitudes) in bacteria. See Lieber, 1980b; Lieber & Persidock, 1983; Lieber, 1989, 1990, 1998b,1998c, 2000, 2001a [and weblinks].
This research predicted problems and implied solutions to such (Lieber, 1989) which are currently being addressed in plant genetics research
(Lieber, 2005). Moreover, the findings, using bacteria, were later confirmed by other scientists. (References are given in my publications.)
Such research has begun to open up a whole new way of looking at mutagenesis, especially its relationship to disease and to the development of beneficial crops and trees.
However, to be even more effective, the medical and agricultural sciences require newer, more completed, developed approaches utilizing new and more completed theoretical models or paradigms.
A comprehensive, new theory is needed, not just facets or parts of such. Without new theory, on which scientific progress has been based, the biological sciences will eventually stagnate; consequently, they will be unable to address key problems, calling for creative approaches, thus jeopardizing the future of human progress and development.
Because of my broad experience in the biological
sciences, creativity, and in-depth approach, I have provided and am able to
provide new, effective theoretical perspectives and breakthroughs on research issues in biology, especially as they pertain to mutation, gene function, development, and adaptation. In this way, I could, as a Consultant, provide a valuable service in any research endeavor.
For example, I can provide detailed protocols---as opposed to conventional protocols---that enable a high frequency of plant development (regeneration) from plant neoplasm in culture.
If you want to review such a protocol for a given plant species, this would be provided for at a very reasonable fee.
I can also provide
on-site advice/support
at your laboratory or agency.
For more infornation please refer to Biography
of Michael Lieber.
I can be contacted at Genadyne Consulting, Phone: (510) 526-4224
E-mail: michaellieber@juno.com Michael M. Lieber, Ph.D. [TOP]CAPTIONS for PHOTOGRAPHS Figure 1a - Very small embryonic pine-plantlet (top center) emerging from brown inhibited callus (neoplasm) derived from a pine needle.
Figure 1b - Pine bud emerging from callus derived from a pine needle.
Figure 1c - Pine shoot growing from callus derived from a pine needle.
Figure 1d - Small male pine-cone developed from pine-needle callus.
Figure 1e - Pine shoot growing form callus derived from pine needle.
Figure 2 - Bean plantlets and buds emerging from callus having an immature-embryo source.
Figure 3a - Bean embryos regenerated from somatic callus derived from a shoot apex. Many embryos have produced plantlet-shoots.
Figure 3b - Embryogenesis from bean callus producing bean shoots. Callus was derived from the meristem of a bud apex.
Figure 3c - Embryogenesis from another bean callus poducing bean shoots
Figure 4 - Buds in various developmental stages emerging from callus derived from a shoot apex of the green bean.
Figure 5 - Bean plantlets regenerated from meristemic callus of a shoot apex. Figure 6 - Buds and plantlets regenerated from meristemic bean-callus of a shoot apex.
Figure 7 - An example of hypermutation in the fungus, Aspergillus nidulans, cultured under stress.
Figure 8 - Adaptively responsive hypermutation in Aspergillus cultured under an increased-temperature stress.
Figure 9a - Pine cone displaying seed nodules arranged in logarithmic spirals.
Figure 9b - Bottom of a pine cone displaying a spiral morphology.
Figures - 10a and 10b - Vortical cacti.
Figure 11 - Cactus with a spiral morphology.
Figure 12 - Helical tendril of a vine.
Figure 13 - Two roses, each with a vortical design.
Figure 14 - A gastropod shell with a spiral morphology. [TOP] | Click To Enlarge Photos
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